{APPRIS} is a system that deploys a range of computational methods to provide annotations of alternative splice isoforms and identify principal isoforms for vertebrate species.
The APPRIS principal isoform for each gene is selected as the principal isoform by combining protein structural information, functionally important residues and evidence from cross-species alignments.
The server has been used to annotate the Human genome as part of the GENCODE consortium but {APPRIS} also has annotations for other vertebrate species. The main data source for {APPRIS} are the Ensembl annotations and sequences, but we have increased the gene sets including the RefSeqGene reference sets, and UniProt proteomes. In addition, we have created new gene sets based on the merge of Ensembl+RefSeqGene+UniProtKB isoforms for each gene.
There are three ways to retrieve annotations for alternative splice isoforms:
In both the APPRIS WebServer and the APPRIS WebServices, the annotation and analysis is based on the modules in the pipeline of APPRIS Database.
Clearing the cache helps to fix pages that freeze or don't finish loading or that have error messages. It is also useful in those cases when outdated annotations appear in the web pages.
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